IVF Treatment Provided at Pulse Women's Hospital

Intracytoplasmic Sperm Injection [ ICSI ]

It is the process in which the embryologist, by very precise maneuvers of multiple manipulator devices under highly specialized microscope and specially developed fine glass needle, picks up a single sperm and microinjects it into the cytoplasm of an egg to achieve fertilization.

ICSI procedure consists of the following steps:

  • All mature eggs and sperm are placed separately in a petridish and kept on the stage of micro manipulator.
  • A holding pipette holds and stabilizes the mature egg by applying gentle suction.
  • A sharp, thin  hollow glass micro pipette is used to collect a single sperm after immobilizing it.
  • This pipette is then pierced through the outer layer into the inner part of the egg.
  • The sperm is then released into the egg.
  • After injecting the sperm in each egg , they will be placed back into the culture media to achieve fertilization and subsequently cleavage of the embryos.

Embryo Transfer

This is done on an outpatient basis and does not require anaesthesia. The patient lies on the table and doctor using a vaginal speculum exposes the cervix. Two or three good quality embryos suspended in the culture media are drawn into a transfer catheter. The tip of the loaded catheter is gently guided through the cervix under sonography control and the fluid containing the embryos is deposited into the uterine cavity. This procedure takes about 10 - 15 mins. The patient is advised to take bed rest for a few minutes after transfer. The supernumerary embryos can be cryopreserved and stored in the liquid nitrogen containers for later IVF cycles, if necessary.